Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: TQ Ameliorates Oxidative Stress and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Immunohistochemical staining images of SOD1 in the control and TQ groups (scale bar, 50 μm). (B) Quantitative analysis of SOD1 absorbance in the control and TQ groups. (C) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control and TQ groups. (D) Quantitative analysis of oxidative stress-related protein blots. (E) Immunohistochemical staining images of C-CASP3 in the control and TQ groups (scale bar, 50 μm). (F) Quantitative analysis of C-CASP3 absorbance in the control and TQ groups. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control and TQ groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Immunohistochemical staining, Staining, Control, Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Thymoquinone alleviates the accumulation of ROS and pyroptosis and promotes perforator skin flap survival through SIRT1/NF-κB pathway

doi: 10.3389/fphar.2025.1567762

Figure Lengend Snippet: EX527 Reverses the Inhibitory Effects of TQ on Pyroptosis, Oxidative Stress, and Apoptosis in Rat Perforator Flaps After Ischemia-Reperfusion Injury. (A) Western blotting to detect the expression of pyroptosis-related proteins: NLRP3, GSDMD-N, Caspase-1, IL-1β, IL-18, and ASC in flap tissues of the control, TQ, and TQ+EX527 groups. (B) Quantitative analysis of pyroptosis-related protein blots. (C) GSDMD-N protein expression levels in flap tissues of rats in the control, TQ, and TQ+EX527 groups measured by immunofluorescence (scale bar: 20 µm). (D) Fluorescence intensity of GSDMD-N expression in each group analyzed using Image J. (E) Western blotting to detect the expression of oxidative stress-related proteins: eNOS, HO1, and SOD1 in flap tissues of the control, TQ, and TQ+EX527 groups. (F) Quantitative analysis of oxidative stress-related protein blots. (G) Western blotting to detect the expression of apoptosis-related proteins: Bax, C-CASP3, and Bcl-2 in flap tissues of the control, TQ, and TQ+EX527 groups. (H) Quantitative analysis of apoptosis-related protein blots. Significance: *p < 0.05 and **p < 0.01. Data are presented as mean ± SEM, with five rats per group.

Article Snippet: Anti-cadherin 5 is provided by Wuhan Boster Biological Technology, Ltd. Primary antibodies against GAPDH, HO1, SOD1, Bax, Bcl-2, GSDMD-N, and VEGF are sourced from The Proteintech Group (Chicago, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Fluorescence

Journal:

Article Title: Lifelong Running Reduces Oxidative Stress and Degenerative Changes in the Testes of Mice

doi: 10.1677/JOE-08-0306

Figure Lengend Snippet: Relative quantification of gene specific mRNA by real-time PCR

Article Snippet: Primary antibodies included: rabbit polyclonal against SOD1, rabbit polyclonal against HO-1 and goat polyclonal against GPX1 (Santa Cruz Biotechnology Inc CA); anti-phospho-histone H2AX (Ser139), clone JBW 301 (Upstate, Temecula, CA); rabbit polyclonal against HNE-Michael Adducts, reduced rabbit pAb (Calbiochem, EMD Chemicals Inc. San Diego, CA); and a mouse monoclonal against β-actin (Sigma-Aldrich, St. Louis, MO).

Techniques: Quantitative Proteomics, Sequencing

Journal:

Article Title: Lifelong Running Reduces Oxidative Stress and Degenerative Changes in the Testes of Mice

doi: 10.1677/JOE-08-0306

Figure Lengend Snippet: Lifelong running results in reduced levels of lipid peroxidation, and protein carbonylation and nitration in the testes. Levels of H2O2 (A), isoprostanes (B), nitrotyrosine (C) and protein carbonyls (D) were measured in testicular samples sedentary mice and runners. Values are the mean and SEM (n = 6 mice per group) *p< 0.01. Proteins isolated from testis of sedentary mice and runners were used to measure levels of antioxidant enzymes (SOD, Cu/Zn-superoxide dismutase; HO-1, heme oxygenase; GPX, glutathione peroxidase) and γ-H2AX (E and F) and proteins modified by the lipid peroxidation product 4-hydroxynonenal (HNE) (G and H). Values are the mean and SEM (n = 6 mice per group) *p<0.05. ***p<0.001, **p<0.01. I. mRNA levels of SOD1, SOD2, GPX-1 and GPX-4 in sedentary mice compared to runners. Values are the mean and SEM (n = 6 mice per group). *p<0.001.

Article Snippet: Primary antibodies included: rabbit polyclonal against SOD1, rabbit polyclonal against HO-1 and goat polyclonal against GPX1 (Santa Cruz Biotechnology Inc CA); anti-phospho-histone H2AX (Ser139), clone JBW 301 (Upstate, Temecula, CA); rabbit polyclonal against HNE-Michael Adducts, reduced rabbit pAb (Calbiochem, EMD Chemicals Inc. San Diego, CA); and a mouse monoclonal against β-actin (Sigma-Aldrich, St. Louis, MO).

Techniques: Nitration, Isolation, Modification